You have to have at least LabVIEW 6.1 to use the NI-CAN 2.2 driver. So you
might be OK running it on a Win95 machine.
I didn't find anything saying that it won't work on Wi95, it may not be supported, which just means that if you run into problems, NI support probably will not help you. On the download page for the driver, it says it supports Windows 2000; Windows 98; Windows ME; Windows NT and Windows XP. They may have indeed dropped support for Win95. LabVIEW 7.1 will not even install on a Win98 or 95 machine, so they may have done the same for the new CAN driver. Only way to know for sure is to try it.
Ed