Yes it's possible. The config util "C:\Program Files\National Instruments\NI-CAN\CANConfig.exe" associates each port to a CAN name and then you can reference/use them in 2 separate CAN networks. You can modify examples in LabVIEW, C:\Program Files\National Instruments\LabVIEW 6.1\examples\nican or make calls to the NI-CAN dll.