Hello,
The File I/O in a RT box is very similar to the way is done in a PC. I would suggest you to check a shipping example with LabVIEW for File I/O. There is a very simple one that is called Write to Text File. Just go to Help » Find Examples. Then click on search and type File. Look for this example.
The path to the file would be something as c:\temp\test.txt.
The flash card in the controller would be referenced as the drive c. In case that you have two flash cards, then it will be c: and d: .
It was not very clear what you mean by keep on loading even though the RT box is off. In that case, where you are storing the data is in a different PC or different PXI, right? Anyways, it would be the same thing.
For opening the file at soon as the PXI boots, then just make your VI that the first thing it does, is to read from the file. Look for the shipping example "Read from Text File". Then convert the VI into an application and download it into the RT box. That way every time you boot your PXI, it will execute that application and will load the file info.
Hope this helps.
Ricardo S.
National Instruments.