Hello Mohd,
I'm not sure I understand exactly what you are asking, but I'll take a stab at it.
Are you using an external clock or what would cause you not to acquire the number of samples you had previously specified? The way the acquire functions work, they wait until they have acquired all of the samples you requested before returning them. To return samples from the acquire functions more often you can either increase the rate or decrease the number of samples acquired.
Post again if this is not what you mean and we'll try to give you a hand.
Regards,
Micaela N
National Instruments