Hi Roy,
Making histograms in DIAdem is relatively easy. Once you have imported your data, follow these steps:
01. Go to DIAdem ANALYSIS
02. Choose STATISTICS FUNCTIONS
03. Select CLASSIFICATION (2nd icon from the left)
04. Select "Sample Count" for the Classification method (that is the actual histogram calculation)
05. Click on the CLASSES button and set the parameters. The most important thing here is the "Number of channels" which could also read "Numer of classes" or "Bins". You can set the other parameters as well, but the most important one is the "Number of channels".
06. Click OK and in the dialog, select the channel (column) you wish to analyze.
07. Select how you wish to see the result data in the bins:
absolute = number of actual samples
per bin
relative = percentage of the number of samples per bin of the total - sums up to 100
08. Click OK to execute the calculation
09. Go to DIAdem REPORT
10. Choose a 2D layout
11. Drag and drop "Class mean" as X and "SampleCount" as the Y channel into the 2D axis system
12. Double click inside the 2D layout
13. For the "Display Mode" choose "Bars" and click OK
You should now have a 2D bar graph representing the histogram of the channel you have selected to do the classification for.
Please let me know if you have any further questions.
Otmar
Otmar D. Foehner
