I used DAQmx VI to program the pxi 4472 and pxi 6602 card so that one does the data acquisition the other generate the pulses. I hope these two card can work at the same time, so I specify in the trigger property node that both of the two card will use the star_stigger riging edge as the digital start trigger source and edge, and it can work. But if I changed the trigger property node into start trigger instance of the DAQmx trigger.vi , and specity the digital source and digital source edge, it doesn't work. In theory, I think both of the two methods can work, why in practice it doesn't??