Cherry:
The last-bit and the stop-bit are 2 different things. The last-bit VISA setting lets you basically treat an extra out-of-band data-bit as an EOI character. Some devices may send back the last byte of data with the most significant bit set. So for example, a linefeed without EOI would be 0x0A, as you expect, but a linefeed signifying EOI would be 0x8A.
The stop-bit is a lower level serial thing.
The last-bit setting is not something that a whole lot of devices use, but I do know that they exist.
Also, if you will use this setting, I suggest you use NI-VISA 2.5 because there were some bugs fixed related to the last-bit feature.
Dan Mondrik
Senior Software Engineer, VISA
National Instruments