If you just follow the wire, you should be able to tell what buffer you are using. Just remember that when a wire goes into a subVI, it is doing an operation on the buffer and the buffer is then changed. If you want to go back to the image before that subVI did its operation, then you need to create another buffer BEFORE the operation and copy the image to that second buffer.
If you are having a hard time knowing what state the images in your buffers are in, use a probe and highlight execution mode to debug your program. If you right click on any of the purple image wires and tell it to "create probe", as the data flows through that wire, you will be able to tell what state the image is in.
Creating more buffers is not bad for your system. It just allocates memory for images on RAM. I run 512MB of RAM and I can get about 80-90 32bit RGB image buffers before I get an error.
Nicholas C
National Instruments
Applications Engineering