Antonio,
I don't know much about the PDF 417 however there are some examples on NI's site about generating barcodes in LabVIEW.
How Do I Generate a Barcode in LabVIEW?In addition you can use the IMAQ software etc to read in barcodes if you don't have a scanner. If you do have a scanner then I imagine it has software and some sort of interace that you could use LabVIEW to read data. It may even have a serial connection and you could use all the VISA Serial VIs in LabVIEW to get the data.