Aha! You want to use AI Read in this case, not AI Single Scan. Replace that VI with AI Read, and you'll have to wire an input to tell it how many scans to read from the buffer (I'm assuming 1 seconds' worth of data, since you have a 1 second delay in your loop). If you don't wire that, it will read the entire buffer.
Sometimes you'll get an error when you've started a continuous acquisition, and then try to perform an unbuffered acquisition, like single scan. Make it a common practice to call the AI Clear after your loop is terminated. You may have to run this VI once all the way through to stop the continuous acquisition, and then it will work the next time.
This occurs for me if the VI is running, and then I press the stop button on the LabVIEW too
lbar instead of my stop button on the Front Panel; the acquisition isn't cleared, and the next time I run it (providing LabVIEW was not shut down), I'll get errors like this.
Mark