Hello Mc D,
Generally speaking, if you're VI works in the development environment, but not as an executable on a target machine, there are a few things you can check:
1. Did you install the LabVIEW runtime engine?
2. Did you install any drivers required by your application?
3. Do you have any file paths that are "hard-coded" in your block diagram?
More details would definitely help us sort out what could be going on. Thanks!