Help: error 1074395247 invalid template descriptor.

[DylanFan]

Hello,

I am trying to implement a programming that use webcam to catch video by function Vision Acquisition, and track a small object (shown on picture below) by Vision Assisstant and finally save it to AVI.

The first time I run the programming, it works. However, after I restart my computer, the overlay rectangle does not appear to the imgae in front panel anymore. If I connect error out from Vision Assistant to IMAQ AVI2 Write Frame, the AVI video even can't be saved. 

I have attached my code, would some one help me to figure it out?

Best Regards,

Dylan