How can I plot Z-axis as a color in Intensity Graph

[Debu]

Hi GerdW,

                   Thanks buddy for your help. Could you please let know How can I make a 'big counter', I mean when it's displaying in front panel so that I can see it from a relatively far distance.  I found that it's getting elongated.

  Thank you

Debashis

[Debu]

Hi GerdW,

    Please find my .vi file.  Could you Please let me know how can I visualize all the scanned area ?  I need to keep the visulaization system in that loop so that I can see after each line scanning. But if you look .vi file it's quite celar that the connectivity have some problems and also when for the next line scanning it rplaces the old one.   I found some problem  with gradient setting along  intensity.

Thanks for your anticipation.

Debashis

[CarlaU]

Hi Debu,

Thank you for the VI. I know you have a lot going on in the VI and I was hoping that you could simplify it a bit so you pinpoint exactly what your question is referring too. Thanks for the help!

[Debu]

Hi CarlaU, 

 The algorithm that I want to implement is that:

1. Define Scan range along X and Y. (the scanning will read out in front window, we are using piezo-stage)

         a) For a value of Y :

                       a) Scan X with specified step-size

                       b) each value of X (step size) record the intensity

                       c) Display the image when scanning ends

          b) Move to the next position of Y with a specified step size

                       a) Scan X with specified step-size

                       b) each value of X (step size) record the intensity

                       c) Display the image when scanning ends but keep all the scanning image  ( it should not replaces the previous image)

          c) keeping doing when Y reach the final point and keep displaying all the images

          d) At the end it will ask me to save the all the data.

Important Points:

         1) I need to see the read out in each counter(photodiode) so that I don't blow them.

        2)  Visulaization system needed:

                                                              Z -axis will be intensity and X and Y are scanning region. I want to see them in 2D plot  where color code will show the intensity and X and Y are scaning dimension. It will be much better if I visulaize  then in 3D parametric surface plot posted by Ben. (http://forums.ni.com/ni/board/message?board.id=170&view=by_date_ascending&message.id=282238#M282238). The problem is that I have only one slice. I didn't understand cleary the idea behind 3d parametric plot.

I am new in this field and I hope you guys will help me. Please let me know If you need to know regarding this.

Thank you

Debashis

[Ben]

Your code crashes on my machine so I can only get a glimpse of the code before the crash.

It does not look like you have attempted to incoporate any of the code I posted in the other thread.

You data file is bogus!

According to the XY coordinates recorded, it looks like the probe followed a diagnal until it hit a limit and then follwed that limit until it got stuck in the corner.

In addition, your previous screen shots indiated 100X100 matrix but the file has more than twice that number of points.

So either try to incoporate the changes shown in the other thread I cited or post a real data file.

Ben

[Debu]

HI Ben,

 "Your code crashes on my machine so I can only get a glimpse of the code before the crash".......................

 Could you please tell me what should I do so that you can see my code

"You data file is bogus!"...............That's true, I don't have clear concept of data file.

Yeah, I wanted to attempt your code...........but I didn't understand it properly...Even, I am not getting

"In addition, your previous screen shots indiated 100X100 matrix but the file has more than twice that number of points.

If you scan 0 micron to 100 micron along X  with stepsize 0.5 micron and along Y,  0 micron to 100 micron  with stepsize 1.0 micron..the total number of pixel is 201x101. The total number of Intensity (along Z)=201x101. 

I hope I am able to convey you.

Thank you
Debashis

[Ben]

With those dimensions I did the attached were i assumed that the odd numbered scans were taken the reverse direction.

Please duplicate the code I have illustrated and post better data if you still have trouble.

What physical phenomenon is illustraed by this data?

As 3D

AS Surface

Zoomed in on hot spot

the code

The sub-VI used to convert the colors was from the the other thread I cited. The flip-side of the case in the nested loops uses "NaN" for values less than "0".

Ben

Message Edited by Ben on 03-23-2008 08:45 AM

[Debu]

Thanks Ben,

                   Great. This is something I was looking for. I am looking for single fluorescent 'quantum  dot' using confocal microscopy.

Could you please post the .vi file of the code. I found some probelm with connectivity for w-matrix.

Can you please set a gradient (color) so that it will automatically modified when I will autoscale Z-axis)

One more thing that I would like to know the how can I do the real time imaging (can you please go through the algorithm).

" c) Display the image when scanning ends but keep all the scanning image  ( it should not replaces the previous image)"

  I was unable to do that....because all the time it replaces th previous image. I am attaching a scheme which will basically tells you the basic differences between what ia m looking for and what was there.

Thanks for your help.

Debashis 

[Ben]

I and all of the other "gold bar" contributors on this forum are volunteers who are just trying to help others learn LV.

I have seen no evidence that you are trying.

"Give a man a fish and he'll eat for a day. Teach an man to fish and he'll eat for a life-time."

If I do your work for you, you will never learn.

Ben

[Debu]

That's True.....I fully agree with you.....You know, I was exposed to NI software no more than 2 weeks. I was learning steadily...I hope one day I will be able to upgrade my system. evryone will probably agree with me that at the initial stage there are several problems that a person can face. My only request you is that you can suggest me some guidelines for moving forward.

I should thank all the contributors in this thread.

Debashis