In your top-level VI, under VI Properties>Documentation there is place for a "Help path". You can create and save an html document and put the link to this document in the Help path. While the application is running the operator can press F1 and the default web browser will open this link. I've written software user guides in Word, saved a copy as an html and linked to it in the Help path.
Dennis, did you work for Aspen Test Eng? If so did you do some programming for QualMark for their test chambers? I have some LabView questions for someone that knows about QualMarks hardware.