If you have the app builder, create an exe and an installer. The installer will put the LabVIEW run-time engine on the other PC. If you require any hardware such as DAQ or GPIB, you will also need to install the hardware and the drivers for them on the other pc (i.e. NI-DAQ, NI-GPIB, NI-VISA). If you don't have the app builder, the other pc will require a licensed installation of the LabVIEW development system. Then you can go to Save With Options>Development Distribution. This will create an LLB that contains your top-level main VI and all subVIs.
