Hi Jamie,
1. Do you have NI-VISA driver installed on the machine on which you are running this executable?? If you are trying to run the executable on the same machine on which the development program has ran fine, then you can ignore this point.
2. If you have done all the changes that are suggested by other discussions related to this topic, then try changing the Port input to Visa Serial Configure.Vi from a control to a constant and try it. In some case, I have seen this to do the trick. I think this point should solve your problem. If it does do tell me. :-))