First of all, I would recommend upgrading to LabVIEW 6i and Vision Builder 6.0. The reason I would recommend this, is that the new version of Vision Builder will automatically generate your LabVIEW code for you. This could make your life much easier.
All you would have to do is build your script using Machine Vision Pattern Matching. This algorithm will compare your acquired image to a template (the image on disk) and return the X and Y coordinates of the pattern match, as well as a score of the match. This score ranges from 0-1000, so you can easily calculate the percentage of resemblance.
Once you have your script working in Vision Builder, you can just go to the Script menu and select Create LabVIEW VI... and it will open up LabVIEW and create the program for y
ou. If you decide not to upgrade to Vision Builder 6, you can get a list of instructions to build your VI by going to the Script menu and selecting Builder File. Hope this information helps.