02-15-2011 11:55 AM
So here's what I have so far written, I have LabView program to interface with my microscope stage, autofocus. I want to try now to interface the camera using Vision Assistant.
The procedure I want the program to perform is taking this CR-39 detectors and placing them on this stage and the camera to take images of this detector frame by frame. So I want the program to go to the very top corner of the detector on the stage. Then the camera would autofocus (if needed) and take an image. So this would take an image for every micron of the detector on that row. Then move to the next and so on. Until it has successfully imaged the whole detector. Is anyone well versed on Vision Assistant and willing to give some advice on this? It would be greatly appreciated.
The purpose is eliminate the previous method of acquiring images by manually focusing on the tracks created by the particles. I want to this program to run itself without a user being present, so just pressing a button would send the program into autopilot and it would perform the procedure.
What are CR-39 detectors?
Allyl Diglycol Carbonate (ADC) is a plastic polymer. When ionizing radiation passes through the CR-39 it breaks the chemical bonds of the polymer and it creates a latent damage trail along the trajectory of the particles. These trails are further ethced using a chemical solution to make the tracts larger which can finally be seeing using an optical microscope.
Thank you,
HumanCondition
02-16-2011 05:45 PM
Hi HumanCondition,
So you already have a vi that can control the stage, correct?
And now you are looking to control the camera? You say you want the camera to "go to the very top corner" and take images frame by frame. Does this mean you want to move the stage, then take an image. Then you want to move the stage again and then take another picture?
You also mentioned auto focus. Is this a control on the camera or are you moving the stage up and down to focus?
02-17-2011 12:54 PM
Hi Justin,
Yes I already have a vi that controls the stage.
So the camera is stationary above the stage. Now what I want to do is get the camera to take the pictures of the detector by moving the stage frame by frame for example starting from the top right corner of the detector (by taking an image) then ending on the top left corner of the detector (by taking an image) then having the stage move down a row and starting the process over again until each frame (or piece) has been imaged.
My goal is while the stage is moving to every little frame of the detector I would like for it to autofocus if necessary, and take a image to be for each frame of the detector.
Using the auto focus requires the moving of the stage up and down.
HumanCondition
02-18-2011 04:15 PM
Hi HumanCondition,
I think what you would need to do is some sort of pattern matching or geometric matching. However, this would only work if the object you are imaging is the same shape each time. I'm not quite sure what these CR-39 detectors are. Would you be able to post a sample image?
Thanks,
02-20-2011 11:22 PM
Hi Justin,
I will have the sample of the CR-39 up later, but okay let's start with the basics. So what would if I wanted to image the detector but only want the first row imaged. Or a good collection of images taken so I can save those photos put them on a usb and study them for later use. For the moment forgeting about looking for specific shapes how could I program my camera to take the images no matter the size of the detector whether it is oddly shaped or not? and also moving the stage at the same time?
Thanks,
HumanCondition
02-22-2011 11:06 AM
It sounds like you would want to program the stage to move over one frame and snap an image. Then move the stage another frame and take another image. I would recommend starting with one of the LabVIEW examples such as snap.vi or snap (Express).vi (These are located in the Example Finder under Hardware Input and Output>IMAQdx>High-Level).
When your stage has moved to the correct location, you can snap an image and then save it if you'd like. Then have the stage move to the next location and snap another image.