It's worked fine on all of our XP boxes and win 2k as well. The older NI-DAQs, however, do not, so you can't use the old ISA legacy boards (not an issue if you're using a laptop). You'll likely have a harder time getting laptop specific drivers than any labview problems. Also, there can be complications regarding file privaledges and labview, such as a limited user trying to run an admin created file, which you can search for solutions for on the discussion forum or ni.com.
2006 Ultimate LabVIEW G-eek.