AO Single Update will perform a *single* update. There is, and can not be, any timing involved since it only happens once. Using AO Clock Config with AO Single Update does not make sense. This is why you will always see it with AO Write.
If you wish to calculate your value and write the same value multiple times then use AO Clock Config and AO Write. If you wish to calculate your value and wait a specific period of time before writing it, for example calculate a value and wait 5 seconds until you write, then you will have to use AO Single Update with a Wait VI.
Regards,
Justin Britten
Applications Engineer
National Instruments