Yes, the Save to File window will open every time. To eliminate this, you can use something called a simple state machine. There should be an example in LabVIEW under "Find Examples". You can search for "state" or "event handler". I'm not sure that that would even work very well necessarily. When you acquire the data, it has to go somewhere. Right now instead of filling up a buffer, it gets written to file. To do what you want, if I'm thinking correctly, you'd have to either (a)keep the data in the buffer and then access it later to save it to file, or (b) write it to a temporary file each time, then when you want to "save" the data to another file, it just reads from the temp file and wr
ites to the new one. Does that make sense? It's probably not as complicated as I'm making it seem, I've just never done it.
Secondly, here's a file that uses the Format Date/Time String in Labview. You can get fractional seconds to whatever precision you want. I have it set on two. Rather than the format HH:MM:SS:ss, it is HH:MM:SS.ss
I haven't seen too many posts that give you the format you want, so sorry that I dont know how to do it.