Hi,
You fail to mention what, if any, error you get and what version of NI-DAQ you are using?, so I will assume you're running into the classical problem using version prior to 7.3.
See the following link for KB describing this known issue:
Multiple DAQ AssistantsRegarding your second issue - could you be a little more specific to what is meant by AC coupling? What kind of AO are you seting up using the DAQ Assistant?
Regards
Anders M
National Instruments DK