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particle detection with grid background

I am attempting to detect cells in an image from a microscope, but the problem is that a grid background exists which prevents the typical thresholding method to work. I have tried using a low pass filter to remove the grid pattern, but in order to filter out the grid completely, the cells become too blurred. An example image is attached. Is there any function to filter away the grid while keeping the cells in focus?

 

There are also light patterns on the image, but a color plane extraction already removes their intrusion on thresholding.

 

The ideas mentioned in the thread below may help to detect the cells after the grid has been removed.

http://forums.ni.com/t5/LabVIEW/particle-detection-with-poor-background/td-p/679618

 

Thanks a lot!

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Hello Tiraken,

 

One potential solution is to take an image of the grid without any cell then subtract all images with the template.

Andy Chang
National Instruments
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