How is the data stored? What function did you use to write it? There are several functions for reading files - Read From Spreadsheet file, Read Waveform from File, Read Lines From File, Read LabVIEW Measurement File, Read Characters From File, etc. Provide some more details or you can attach a copy of a data file so that someone can provide a little more help. Basically, you need to create to extract the Y values into an array and get the sampling rate from the file. Once you've done that, you can pass the information to an analysis routine as a waveform or signals data type.