If you're looking at how to control the handshaking lines of a serial connection, the exact method will depend on which functions you want to use for basic serial communication. There are the older serial compatiblity functions. VIs with these are a little easier to distribute as executables and are a bit smaller. There is a VI called serial line control.vi in the \vi.lib\platform\_sersup.llb. There have some recent questions about the future of the serial compatiblity VIs in favor of just having the VISA functions. You control the handshaking lines in VISA with a VISA property node. The lines are selectable under the Modem Line Settings property.
To give you more details, you need to provide more information such as pulse width, line you want to use
, how you're acquiring the temperature.
