Rebecca,
Yes, you can use the same PFI line as both a start and a stop trigger for acquisition. I have included a link to an example program below that illustrates how to do this. I hope this helps.
Continuously Acquiring Analog Signals Using a Digital Start and Stop Trigger
http://venus.ni.com/stage/we/niepd_web_display.DISPLAY_EPD4?p_guid=B123AE0CBB00111EE034080020E74861&p_node=DZ52308&p_submitted=N&p_rank=&p_answer=&p_source=Internal
Regards,
Todd D.
Applications Engineer
National Instruments