Hi jimmy1981,
I would recommend making your subVI's re-entrant if you need to have multiple different instances of the same VI. For more information on this topic, please take a look
here.
If you have questions about the best method for calling your sub-VI, take a look at
this KnowledgeBase. It describes the differences between a couple of sub-VI calling methods.
Please let me know if you have any further questions.
Regards,
Ching P.
DAQ and Academic Hardware R&D
National Instruments