the strategy by which each VI's component wok how they bring out the essential detection and validation results

[PreethiGovindarajan]

Hello all,

How to find out the strategy by which each VI's component work how they bring out the essential detection and validation results, basically the exalanatio of each vi compoent in the functions palette the programming palette of the block dagram and front panel.

[Bob_Schor]

I'm not sure I know what you are asking, but I think that if you spent some time with the LabVIEW Tutorials, and "played" a bit with LabVIEW  by trying to build on what the Tutorials are trying to show you.  Experiment, try things, you'll begin to figure out (for yourself) how things work.

Bob Schor

[PreethiGovindarajan]

Hello,

Bob_Schor

I am  attaching the Vi file in which I want to incorporqate the shape detection between the nucleus and a cell and also the overall area and the no. of cells and pixels occupied.

[Bob_Schor]

That makes a lot more sense.  Why don't you see if you can ask the Moderator to change your Title to something like "How do I use NI Vision to analyze images of biological cells?".

Since I see you are using Image Assistant and what looks like "canned NI code", I'm assuming that you have little experience with NI Vision and its tools.  How much do you know about Image Analysis?  Do you know about edge detection, particle analysis, pattern matching, all those little "in things" that are not always well-documented in the NI Vision functions?

How much general LabVIEW Experience do you have?  Are you "starting from scratch", or have you been doing LabVIEW Development for several years?

What specific questions do you have?

Bob Schor

[PreethiGovindarajan]

I have been using LabVIEW for some years for Signal acquisition and analysis but image analysis this is my first if you help me out in counting the number of cells and detect a nuclei form a cell then I guess take this project forward. You might see vision assistant and stuff I just tried by reading some  context help of the IMAQ Tools.

[Bob_Schor]

My advice would be to look at the Examples that ship with LabVIEW, see if any are appropriate.  There are a few books on IMAQ and LabVIEW Vision, several written more than a decade ago, one about 2-3 years old.  They aren't bad, but don't always explain very much.

LabVIEW Vision (in my experience) is definitely an area where you need to "do experiments" (i.e. write tiny routines that try to do one thing, see if it works, and then (for goodness sake!) document it.  You're largely going to need to teach yourself, so write neat (meaning precise, documented, lots of notes) code, write simple code, figure it out and teach yourself.  If you can find a LabVIEW guru near you with whom to work, it will go much faster.

Bob Schor