Greetings,
With the PCI-DIO-32HS, the software will use DMA transfers by default. The 653X manual has more information about DMA transfers with regards to the PCI-DIO-32HS on page E-2. The manual is available at the following link:
http://digital.ni.com/manuals.nsf/websearch/EDE443F5C700373A862569B90072D26F?OpenDocument&node=132100_US
If you have further questions, let me know. Good luck with your application.
Spencer S.