You could try getting the latest runtime engine and see if that helps. You can get it at: http://digital.ni.com/softlib.nsf/websearch/2F431002B28323BB862569A40056CE28?OpenDocument.
We haven't tested 4.0.1 with W2000 since it came out 4 years before that OS. CVI 5.0.1 and above are known to work fine though.
Best Regards,
Chris Matthews
Measurement Studio Support Manager