Christian -
I would first recommend an article on our Developer Zone about Spatial Calibration. It also has some links if you would like more detail into it.
http://zone.ni.com/devzone/conceptd.nsf/webmain/EF66EEB671B0019B862569D10078C45E?OpenDocument
The next thing to do is to open up one of the Calibration examples for CVI which comes with Vision. You can find the examples in this directory:
C:\Program Files\National Instruments\MeasurementStudio\CVI\samples\Vision\2. Functions\Calibration
I would recommend starting with the Simple Calibration example.
Jack Arnold
Application Engineer
National Instruments