Auto tresholding + Image substraction

[aileenland]

Dear All,

I am a relatively new user of NI Vision Assistant and I use this programme to analyze growing microcolonies of E.coli. Due to the limited explanation in the Help section, I am wondering if any of you can help me in doing :
a. Image substraction from another image (grayscale)
b. Overlapping two images that is not yet 100% aligned to each other
c. Programming my own auto-tresholding. What I would like to have is an autotresholding that have max and min limit on the left side of the peak (from the grayscale vs number of pixel graphs). Does any of you know if this is even possible?

Thanks a lot in advance...

[KvZ]

For image substraction you first need to store the image in a image buffer and then use a operation to subtract them. see example script.

For the Auto trashold, the standard trashold of the Image assistand has some predified auto trashold options, if you wish to modify this, you will need to generate for example LabVIEW code and program it your self.

The same is true for overlaying images, this is not a feature in Image Assistand and needs to be programmed in code.

Met vriendelijke groet / Kind regards,

Karsten
Applications Engineer
National Instruments