Fusing fluorescent images?

[Fantazer]

Hello!

I'm interested if there is any LabView/Vision coda available that will help me ti create fused fluorescent images similar to the one shown in "Automating Fluorescent Imaging Techniques" topic:

 http://zone.ni.com/devzone/conceptd.nsf/webmain/7B4B32925E15F27586256EDF0059A331

Basically, I need to merge several gray scale images assigning a unique color for each image. I would appreciate any help. Thanks.

Pavel

[BruceAmmons]

The quick and easy method will work for up to three colors.  You could use each gray image as the input for a color plane (RGB) when combining color planes into a color image.

Slightly more difficult would be scaling each gray image by the three RGB components of the color you want it to be, then adding them together.  For example, if you want cyan, it is scaled 0 R, 1 G, 1 B.  If you want orange, it is 1 R, 0.5 G, 0 B.  Add all the R images together, all the G images together, and all the B images together, then use them as the color plane inputs as above.

Bruce

[Fantazer]

Bruce,

Thanks a lot for your suggestions. I'm surprized that I was not able to find examples for such procedures because it is relatively popular operation.

Pavel