It is fairly easy.
The first step is to figure out what coordinates you want to use. You can use Find Edge to figure out where the edges of the screen are, and line intersections will tell you where the corners are.
If you just want a simple rectangle ROI, just use rectangle to ROI in the ROI palette. If you want a general polygon, you can create your own version of the same vi.
If there is a high contrast between the screen and its surroundings, another possibility is thresholding the screen from the background, filling any spots, and using Mask to ROI.
Bruce
Bruce Ammons
Ammons Engineering