Classical Physics tells us that velocity is distance traveled over time (speed) and acceleration is the change in velocity over time.
Assuming that your particles are not moving in a vector that is toward or away from the camera (the Z-plane), and also assuming that the frame rate is constant, it should be fairly straight-forward. Each frame is a certain time from the previous frame, so you can easily find the time. If you can locate a particular particle in successive frames, you can take the change in position (distance traveled) divided by the delta T from frame to frame. This should give velocity.
Acceleration is the change in velocity. The easy way to calculate that with this type of setup is to just take successive delta Vs and divide the time.
Now, if either of my assumptions is wrong, it becomes much harder. If there is travel in the Z-plane it becomes very difficult to figure out. Also if the frame rate is not a constant, you will have to find some way to calculate the change in time.
And if you cannot differentiate your particles, I have no idea how it would work.
I hope that this helps,
Bob