How to fill hole effectively?

[samivan88]

Hi everyone. I'm trying to do particle analysis. however, after thresholding, the particles got holes inside them. i've tried to use IMAQ FillHole befor morph, however, the image turns all black. i really hope that the holes can be filled, so that i can morph the particles without losing the information (if there is holes, we cannot apply morph too many times, else, we will lose the information). Thanks in advance! Regards.

[BruceAmmons]

Most likely the Fill Holes routine converts the image to values of 0 and 1.  If you change the display palette to binary, the objects will be red.  Binary Morph will work fine on this image.

Bruce

[samivan88]

thanx for your reply. i already convert my image to binary using autoBthreshold, with method inter-class variance (otsu thesholding). you can see a simplified verssion of my program in the attachment. are they different from what you said?

thank you,

regards,

sam.

[samivan88]

sorry no attachment in prev reply. this is