The equations for Inter-class variance for AutoBinary Thershold are simialr to the Clustering technique described in the Vision Concepts Manual
http://search.ni.com/query.html?col=alldocs&qp=%2Bcontenttype%3Aproductmanual&layout=ProductManual&qt=Vision+Concepts+Manual
Particle Orientation example,
http://search.ni.com/query.html?col=alldocs&layout=Example&qp=ContentType:Example&qt=particle+orientation
I would suggest upgrading to Vbuilder 6.1, you can try a demo at,
http://sine.ni.com/apps/we/nioc.vp?cid=1301〈=US
and also update your framegrabber driver,
http://digital.ni.com/softlib.nsf/webcategories/85256410006C055586256BE60070436D?opendocument&node=132060_US