Tchaou--
I have taken a look at the VI picture that you provided. In order to do the exact same thing your VI is doing right from VBAI you could just use the simulate acquisition step. This will simply load a file from the harddrive.
If you still would like to use a Run VI step for another purpose or were just testing things out, you can still do this. In order to pass an image from a VI to VBAI you need to give it the buffer location that VBAI is using. So you still need to load the simulate acquisition step, this will create a buffer. Then in your VI, on the IMAQ create, add the buffer as a control so that it shows up on the front panel. Then in VBAI when you reference the Run VI step you can configure a control to pull info from the VBAI script. After you click configure, then set that control to "use measurement" and select the simulate acquisition or current image. Then when you pass the image back from the VI it will show up in this buffer and you can continue your VBAI script.
That should work out, but if you have any questions just let us know.
Regards,
John H
Applications Engineer
National Instruments