I believe you can load and save the overlay information along with the image within LabVIEW using "IMAQ Read Image and Vision Info" (and Write).
I don't know of any way to maintain the overlay information outside of LabVIEW. Currently, there is no way to merge it with your image. This would be very useful for printing, or image manipulation outside of LabVIEW.
Bruce
Bruce Ammons
Ammons Engineering