Normally you cannot save this information without this function. This information saved in *.png file. This format have a possibilities for saving "user data", and this fact used by IMAQ Vision. Theoretically you can extract this information from *.png into array (format of png is a well known format), but what can you do with this information afterwards? You cannot load this information separately without IMAQ Read Image and Vision Info.vi, because (pretty sure) this function allocated memory for Vision Info before loading, but you not able to do this. You can make this only if you know internal representation of IMAQ image in memory. How organized common parameters, such as width, height, pixel pointer, resolution, linewidth - not very complicated (IMAQ Image -
a cluster of string and pointer to appropriate structure), but where placed vision info - not very easy.
Better, fastest and easyest way - to use IMAQ Write Image and Vision Info.vi.
with best regards