If you feel comfortable using the Low Level functions I would personally recommend using them instead of the High Level. Bruce's answer is correct, high level will call low level with standard options selected. If you think of ways to improve the speed of your code, you may want to use low level, my reason is because high level VIs will actually be a function call calling other functions, which introduces a minimum delay, instead, if you use low level you are directly calling the functions for acquisition so there is kind of a small time saving if you think of it as acquisition wise.
Also, the high level VIs if you check their block diagram it has more structures for error checking, session ID checking, etc., so with low level you have direct control over the acquisitio
n from your main program without introducing innecessary structures or functions.
Regards,
Nestor Sanchez
IMAQ/Motion Support
National Instruments
Nestor