Hi Spencerch...
May I reformulate your question (to be shure I understood it) : you are taking a serie of 1000 images and you want to locate in it the position of a 2x2 pixel bright spot that corresponds to you laser spot and save this to a file :
wire the "new image" VI to a loop. In the loop wire the image to the "grab image" VI (you have to use the list vi to get the name of the file), then wire it to "Imaq threshold" VI with a constant value of 125 (if bright spot is saturated), then wire it to "IMAQ Particle Analysis " VI ans select in the input "center of mass X" and "center of mass y" and there you get your position .
Cheers.
Message Edité par DOC-doc le 02-02-2007 10:21 AM