What model of camera are you using? Typically, if you use a GigE thermal camera with IMAQdx, you will acquire a grayscale image from the camera, where the pixel intensity value can be directly correlated to a temperature value. Once you have this correlation, you can process only the pixels in an ROI, and get an mean temperature, max, min, median, etc.
Where in the process are you having difficulty? In the conversion of pixel intensity to temperature? In the processing of an ROI?
