The DIO lines on the E Series devices have a 50 kohm pull-up resistor between the digital lines and +5 V. Therefore, you might have to take the size of this resistor into consideration when connecting to circuitry and when you are not actively driving the digital line. If you would like the digital lines to be pulled-low instead of being pulled-high (when the lines aren't being driven) you can add a resistor between the digital lines and digital ground. The document linked below describes how to accomplish this. I hope this helps.
Setting the Default State of Digital Lines on E Series DAQ Boards
http://digital.ni.com/public.nsf/websearch/B4FED0C879BD155F862569ED00142DC7?OpenDocument
Regards,
Todd D.
NI Applications Engineer