Hey Gopal,
Unfortunately you have run into a bug within TestStand. When you try and do partly default values of controls that are on a tab control, this is not handled properly within TestStand.
I have attached an updated LabVIEW library that I believe should fix the problem. Give this a try and let me know. I would recommend making a backup of your TestStand - Default Values xx.llb where xx is the version of LabVIEW you are using. I have attached a version of this library in an older version of LabVIEW to make sure you can open it. You will need to mass compile and rename the library to correspond to whatever version of LabVIEW you are using. It needs to be placed within the <TestStand>AdapterSupport\LabVIEW where <TestStand> is the default installation directory for TestStand.
Regarding your second question about unloading the VI, there are several different ways to do it. Usually the default within TestStand is to unload the VI after the sequence file is closed so normally you won't even have to worry about unloading them. If you want to manually unload all the VIs, you can go to File>>Unload All Modules. Also if you want to edit a specific VI, you can right click on the step that uses that VI and select Edit VI to unreserve the VI. Hope this helps!
Pat P.
Software Engineer
National Instruments