To briefly answer your questions, the particular benefit of FAT32 when
thinking about Virtual Instrumentation systems is that it allows you to
run Real-Time (LVRT or CVI RT) as a dual-boot with Windows. The NTFS
filesystem is not recognized by the RT system. For more information:
Dual-booting
LVRT and Windows
NTFS is a more modern OS. It has some peformance benefits and
additionally allows you to compress, encrypt and add file permissions
to files and directories. This is the OS that NI primarily uses for our
Controllers.