ah, i found the problem.
If you probe on the signals, you'll see a nice 5kHz clock going into strobe but a 50us pulsewidth trigger going into PFI. What's happening is your trigger is asserting and deasserting within a single clock cycle so the trigger gets missed on the next active strobe edge.
Change your data pattern in case "0,default" to
100
110
111
111
this will make sure your trigger is asserted through a clock edge which will trigger the acquisition
Just keep in mind that synchronous triggers are sample in the same fashion as data so they have to meet all of the same setup and hold specifications relative to strobe.
Message Edited by Ryan M on 07-31-2009 09:14 AM