Greetings,
Unfortunately, this configuration is not absolutely reliable. The NI 6533 can stop acquiring data and de-assert the ACK line at any time. Unless you monitor the ACK line, it is likely that you will lose data and not know about it. To perform pattern input at 16 MHz, you may want to consider the NI 655x or NI 6534.
Good luck with your application.
Spencer S.