Zoran,
The REQ signal's polarity can be modified with Digital Mode Config.vi in LabVIEW or the DIG_Group_Mode() NI-DAQ function. This is discussed in further detail in the following KnowledgeBase:
How Do I Change the Polarity of the REQ Line in Pattern Generation on the DIO-32HS?However, the REQ signal is the pattern I/O operation's clock, not start trigger. The ACK signal functions as the start trigger. The same VI and function mentioned previously are also used to modify the ACK signal's polarity.
Good luck with your application.
Spencer S.