The extra command bytes that are sent out during the IEEE 488.1-2003
non-interlocked handshake transfers (HS-488) should not affect any
instruments. However, to be extra safe, NI requires that HS488 be
enabled before it sends out any new commands. If you don't enable
HS488, the board will operate in a mode that is 100% compatible with
prior IEEE 488.1 standards. FYI, to enable HS488, you change the HS
cable length to a non-zero value.